Basic cell culture protocols free download

Images Donate icon An illustration of a heart shape Donate Ellipses icon An illustration of text ellipses. Basic cell culture protocols Item Preview. EMBED for wordpress. Want more? Advanced embedding details, examples, and help! Revised edition of Animal cell culture, published in Mode of access: Internet. Donor internetarchivebookdrive Edition 2.

About this book Introduction In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem ES cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells.

Additional chapters describe new techniques leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells and detail quality control methods for cell lines detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis.

Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.

DNA Macrophages cell cell culture cell lines development enzyme human cell lines neural stem cells stem cell stem cells tissue. Editors and affiliations. Cheryl D. Helgason 1 Cindy L. Miller 2 1. StemCell Technologies Inc. Vancouver Canada. Reviews "This book is written by experienced authors who understand the practical needs of bench scientists. All laboratory personnel who perform cell culture on a regular basis will benefit from it.

The authors provide summaries, keywords, helpful introductions, lists of necessary materials and equipment, and detailed protocols for culturing a wide-variety of cell types. The chapters on manipulating pluripotent stem cells will be of great value to researchers interested in regenerative medicine and cell engineering. This book is an important and valuable contribution to the field of cell biology and the art of manipulating cells in culture.

Author : R. It aims to provide basic instruction in the basic procedures of cell culture for newcomers to the field, including aseptic technique, safety and regulatory issues, equipment and materials, media preparation and sterilization, primary culture, propagated cell lines, characterization and authentication, contamination, cryopreservation, and quantitation.

There are also a number of specialized protocols some of which have general interest, e. Some specialized protocols will be retained in the printed copy but others will be presented in electronic form only, depending on the anticipated readership.

A number of minireviews, some by the author external review and some by invited authors will be added to give an overview of the applications of cell culture. New approaches and procedures have become available and new issues have arisen which require sections of the book to be updated. The increasing diversity of the applications of cell culture also need a revision of how certain topics are presented.

The proliferation of specialized techniques requires that some of these now be presented online to avoid a further increase in size of the book.

In addition the introduction of new topics requires that some of these be presented in mini-review form. Three reviewing editors have been appointed to advise on recent developments and trends and this will help to reshape the book in line with cutrrent demand.

Some new features: There will be a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling existing and barcoding new. Some specialized protocols, e. This edition will focus more on more generally used techiniques and make other less used techniques available online.

New mini-reviews will give insight into newer applications. More emphasis will be given to authentication and problems of misidentification. Illustrations will be updated as required. Explaining both how to do tissue culture and why a technique is done in a particular way, this classic text covers the biology of cultured cells, how to select media and substrates, regulatory requirements, laboratory protocols, aseptic technique, experimental manipulation of animal cells, and much more.

A brand-new chapter examines the origin and evolution of cell lines, joined by a dedicated chapter on irreproducible research, its causes, and the importance of reproducibility and good cell culture practice.

Throughout the book, updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing.

This item is printed on demand. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs.